SWP73 Subunits of Arabidopsis SWI/SNF Chromatin Remodeling Complexes Play Distinct Roles in Leaf and Flower Development

Sebastian P. Sacharowski , Dominika M. Gratkowska , Elżbieta Sarnowska , Paulina Kondrak , Iga Jancewicz , Aimone Porri , Ernest Bucior , Anna T. Rolicka , Rainer Franzen , Justyna Kowalczyk , Katarzyna Pawlikowska , Bruno Huettel , Stefano Torti , Elmon Schmelzer , George Coupland , Andrzej Jerzmanowski , Csaba Koncz , Tomasz Sarnowski

Abstract

Arabidopsis thaliana SWP73A and SWP73B are homologs of mammalian BRAHMA-associated factors (BAF60s) that tether SWITCH/SUCROSE NONFERMENTING chromatin remodeling complexes to transcription factors of genes regulating various cell differentiation pathways. Here, we show that Arabidopsis thaliana SWP73s modulate several important developmental pathways. While undergoing normal vegetative development, swp73a mutants display reduced expression of FLOWERING LOCUS C and early flowering in short days. By contrast, swp73b mutants are characterized by retarded growth, severe defects in leaf and flower development, delayed flowering, and male sterility. MNase-Seq, transcript profiling, and ChIP-Seq studies demonstrate that SWP73B binds the promoters of ASYMMETRIC LEAVES1 and 2, KANADI1 and 3, and YABBY2, 3, and 5 genes, which regulate leaf development and show coordinately altered transcription in swp73b plants. Lack of SWP73B alters the expression patterns of APETALA1, APETALA3, and the MADS box gene AGL24, whereas other floral organ identity genes show reduced expression correlating with defects in flower development. Consistently, SWP73B binds to the promoter regions of APETALA1 and 3, SEPALLATA3, LEAFY, UNUSUAL FLORAL ORGANS, TERMINAL FLOWER1, AGAMOUS-LIKE24, and SUPPRESSOR OF CONSTANS OVEREXPRESSION1 genes, and the swp73b mutation alters nucleosome occupancy on most of these loci. In conclusion, SWP73B acts as important modulator of major developmental pathways, while SWP73A functions in flowering time control.
Publication typeOriginal work published in-full
Author Sebastian P. Sacharowski
Sebastian P. Sacharowski,,
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, Dominika M. Gratkowska
Dominika M. Gratkowska,,
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, Elżbieta Sarnowska (COIW / ZOMT / ZOMT-PB)
Elżbieta Sarnowska,,
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, Paulina Kondrak
Paulina Kondrak,,
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, Iga Jancewicz (COIW / ZOMT)
Iga Jancewicz,,
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, Aimone Porri
Aimone Porri,,
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, Ernest Bucior
Ernest Bucior,,
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, Anna T. Rolicka
Anna T. Rolicka,,
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, Rainer Franzen
Rainer Franzen,,
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, Justyna Kowalczyk
Justyna Kowalczyk,,
-
et al.`
Journal seriesPlant Cell, ISSN 1040-4651, (A 45 pkt)
Issue year2015
Vol27
No7
Pages1889-1906
Publication size in sheets0.85
DOIDOI:10.1105/tpc.15.00233
URL http://www.plantcell.org/content/27/7/1889
Languageen angielski
Score (nominal)45
ScoreMinisterial score = 45.0, 20-09-2019, ArticleFromJournal
Ministerial score (2013-2016) = 45.0, 20-09-2019, ArticleFromJournal
Citation count*1 (2016-03-10)
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